INTERMITTENT FASTING WITH 72 HOURS FASTING AND 96 HOURS REFEEDING PROMOTES FAT OXIDATION AND WEIGHT SUPPRESSION. (June 2018)

• Record Type: Journal Article
• Authors: Endo, S.; Miyashita, K.; Uto, A.; Ryuzaki, M.; Inoue, H.; Fujii, K.; Hiroshi, I.
• Is Part Of: Journal of hypertension. Volume 36(2018)Supplement 1
• Publication Date: 2018-06
• Subjects: Hypertension -- Periodicals; Hypertension -- Periodicals; 616.132005
• DOI: 10.1097/01.hjh.0000539531.58094.25
• Languages: English
• ISSNs: 1473-5598

Abstract

Abstract : Objective: Intermittent fasting (IF) has been shown to have benefits in increasing insulin sensitivity, stress resistance and lowering blood pressure. However, the underlining mechanism for the beneficial effects has not been elucidated yet. Design and method: Mice were subjected to a severe IF regimen, in which they were fasted for 72 hours and then refed ad libitum for subsequent 96 hours. This regimen was consecutively repeated 4 times to enforce the effects. After 4 courses of the IF regimen, mice were fed a high-fat diet (HFD) ad-libitum for subsequent 4 weeks of observational period. The body weight (BW) was monitored throughout the study period in the IF and ad-libitum feeding groups. At the 2 different time points: just after 4 courses of the IF regimen and after subsequent 4 weeks of observational ad-libitum feeding period, the glucose tolerance and respiratory gas were examined. Results: There was no significant difference in the BW between the adlib eating and IF groups just after 4 courses of the IF regimen. Glucose tolerance tests showed a significant decrease in the glucose level of IF group. Respiratory gas analysis showed a significant increase in oxygen uptake and a significant decrease in respiratory quotient of IF group. In PCR analysis, expressions of genes responsible for fat oxidation and thermogenesis (ACO, HSL and UCP1 et al.) were significantly increased in the harvested tissues (the skeletal muscle, white and brown adipose tissues). After subsequent 4 weeks of observational feeding period, the BW in IF group was significantly lower than ad-libitum feeding group. At the time-point, the glucose tolerance, respiratory gas and expression of genes maintained the similar changes observed just after 4 courses of the IF regimen. Conclusions: The 72 hours fasting IF regimen resulted in a suppression of the BW after HFD administration, in addition to a decrease in the blood glucose level. PCR analysis suggested that promotion of fat oxidation and thermogenesis in the skeletal muscle and adipose tissue of the IF group contributed to suppression of the BW.