Multiplex gene editing in rice with simplified CRISPR‐Cpf1 and CRISPR‐Cas9 systems. Issue 8 (7th July 2018)

• Record Type: Journal Article
• Authors: Wang, Mugui; Mao, Yanfei; Lu, Yuming; Wang, Zhidan; Tao, Xiaoping; Zhu, Jian‐Kang
• Is Part Of: Journal of integrative plant biology. Volume 60:Issue 8(2018)
• Page Start: 626
• Page End: 631
• Publication Date: 2018-07-07
• Subjects: Plants -- Periodicals; Plants -- China -- Periodicals; Electronic journals; 580.5
• DOI: 10.1111/jipb.12667
• Languages: English
• ISSNs: 1672-9072

Abstract

Summary: We developed simplified single transcriptional unit (SSTU) CRISPR systems for multiplex gene editing in rice using FnCpf1, LbCpf1 or Cas9, in which the nuclease and its crRNA array are co‐expressed from a single Pol II promoter, without any additional processing machinery. Our SSTU systems are easy to construct and effective in mediating multiplex genome editing. Abstract : We developed the simplified single transcriptional unit (SSTU) CRISPR systems for multiplex gene editing in rice using FnCpf1, LbCpf1 or Cas9, in which the nuclease and its crRNA array are co‐expressed from single Pol II promoter, without any additional processing machinery. SSTU systems are simple and effective in multiplex genome editing.