Highly Stable and Red‐Emitting Nanovesicles Incorporating Lipophilic Diketopyrrolopyrroles for Cell Imaging. Issue 44 (3rd July 2018)

• Record Type: Journal Article
• Authors: Ardizzone, Antonio; Blasi, Davide; Vona, Danilo; Rosspeintner, Arnulf; Punzi, Angela; Altamura, Emiliano; Grimaldi, Natascia; Sala, Santi; Vauthey, Eric; Farinola, Gianluca M.; Ratera, Imma; Ventosa, Nora; Veciana, Jaume
• Is Part Of: Chemistry. Volume 24:Issue 44(2018)
• Page Start: 11386
• Page End: 11392
• Publication Date: 2018-07-03
• Subjects: aggregation -- fluorescence -- imaging agents -- nanoparticles -- vesicles; Chemistry -- Periodicals; 540
• DOI: 10.1002/chem.201801444
• Languages: English
• ISSNs: 0947-6539

Abstract

Abstract: Diketopyrrolopyrroles (DPPs) have recently attracted much interest as very bright and photostable red‐emitting molecules. However, their tendency to form nonfluorescent aggregates in water through the aggregation‐caused quenching (ACQ) effect is a major issue that limits their application under the microscope. Herein, two DPP molecules have been incorporated into the membrane of highly stable and water‐soluble quatsomes (QS; nanovesicles composed of surfactants and sterols), which allow their nanostructuration in water and, at the same time, limits the ACQ effect. The obtained fluorescent organic nanoparticles showed superior structural homogeneity, along with long‐term colloidal and optical stability. A thorough one‐ (1P) and two‐photon (2P) fluorescence characterization revealed the promising photophysical features of these fluorescent nanovesicles, which showed a high 1P and 2P brightness. Finally, the fluorescent QSs were used for the in vitro bioimaging of Saos‐2 osteosarcoma cell lines; this demonstrates their potential as nanomaterials for bioimaging applications. Abstract : Stealth entry : Diketopyrrolopyrrole derivatives are incorporated into the membrane of highly stable and water‐soluble nanovesicles, named quatsomes, leading to water‐soluble fluorescent nanoparticles with long‐term colloidal and optical stability and high one‐ and two‐photon brightness for use in in vitro bioimaging of cells (see figure).