Evaluation of a novel dye for near‐infrared fluorescence delineation of the ureters during laparoscopy. Issue 4 (19th April 2018)

• Record Type: Journal Article
• Authors: Al‐Taher, M.; van den Bos, J.; Schols, R. M.; Kubat, B.; Bouvy, N. D.; Stassen, L. P. S.
• Is Part Of: BJS open. Volume 2:Issue 4(2018)
• Page Start: 254
• Page End: 261
• Publication Date: 2018-04-19
• Subjects: Surgery -- Periodicals; 617.005
• DOI: 10.1002/bjs5.59
• Languages: English
• ISSNs: 2474-9842

Abstract

Abstract : Background: Iatrogenic ureteric injury remains a risk in laparoscopic pelvic procedures. Near‐infrared fluorescence (NIRF) imaging is a promising new technique for enhanced intraoperative visualization of anatomical structures that could improve the safety of laparoscopic surgery. A new dye, IRDye ® 800‐BK, has been developed for intraoperative visualization of the ureters using NIRF. The present study was a first evaluation of the performance of IRDye ® 800‐BK for ureteric imaging during NIRF laparoscopy. Methods: This study consisted of three parts: real‐time in vivo NIRF imaging using IRDye ® 800‐BK in pigs during laparoscopic surgery, ex vivo NIRF imaging of freshly explanted pig ureters and ex vivo NIRF imaging of explanted human ureters. Results: In all animals, both left and right ureters were visualized throughout the laparoscopic procedure for 120 min, with the best results at a dose of 0·15 mg dye per kg bodyweight. NIRF imaging was successful in all human and porcine ureters studied, with a range of dye concentrations. Conclusion: NIRF imaging of the ureters using IRDye ® 800‐BK was used successfully both in vivo in a porcine model, and ex vivo in porcine and human ureters. Abstract : The aim of the present study was a first evaluation of the performance of IRDye ® 800‐BK for ureteric imaging during near‐infrared fluorescence (NIRF) laparoscopy in real‐time in pigs during laparoscopic surgery and ex vivo on freshly explanted pig and human ureters. In all cases successful NIRF imaging was performed. Novel imaging worthy of more investigation.