Antiproliferative Effect of Synadenium grantii Hook f. stems (Euphorbiaceae) and a Rare Phorbol Diterpene Ester. (November 2016)

• Record Type: Journal Article
• Authors: Campos, Adriana; Vendramini-Costa, Débora Barbosa; Longato, Giovanna Barbarini; Zermiani, Tailyn; Ruiz, Ana Lúcia Tasca Gois; de Carvalho, João Ernesto; Pandiella, Atanasio; Cechinel Filho, Valdir
• Is Part Of: International journal of toxicology. Volume 35:Number 6(2016)
• Page Start: 666
• Page End: 671
• Publication Date: 2016-11
• Subjects: Synadenium grantii -- antiproliferative action -- nonpolar fraction -- phorbol ester; Toxicology -- Periodicals; 615.9005
• DOI: 10.1177/1091581816659660
• Languages: English
• ISSNs: 1091-5818

Abstract

Synadenium grantii is frequently used for the treatment of various diseases such as allergies, gastric disorders, and especially cancer. The aim of this study was to evaluate the possible antiproliferative potential of the methanol extract, fractions, and pure compounds from the stems of S grantii . Phytochemical analysis was carried out by conventional chromatographic techniques, and the antiproliferative activity was analyzed using the sulforhodamine B assay and an MTT-based assay. Nonpolar fraction and its subfractions from the stems of S grantii exhibited promising cytostatic effect against several human tumor cell lines (glioma, breast, kidney, and lung), with total grown inhibition values ranging from 0.37 to 2.9 μg/mL. One of the active principles of this plant was identified as a rare phorbol diterpene ester, denoted as 3, 4, 12, 13-tetraacetylphorbol-20-phenylacetate. This compound demonstrated antiproliferative activity against glioma, kidney, lung, and triple-negative breast cancer cell lines. These results demonstrate that S grantii stems produce active principles with relevant antiproliferative potential.