Pressurized hot water extraction of bioactive compounds from artichoke by‐products. Issue 15 (13th June 2018)

• Record Type: Journal Article
• Authors: Pagano, Imma; Piccinelli, Anna Lisa; Celano, Rita; Campone, Luca; Gazzerro, Patrizia; Russo, Mariateresa; Rastrelli, Luca
• Other Names: Ibáñez Elena guestEditor.; Herrero Miguel guestEditor.
• Is Part Of: Electrophoresis. Volume 39:Issue 15(2018)
• Page Start: 1899
• Page End: 1907
• Publication Date: 2018-06-13
• Subjects: Artichoke by‐products -- Bracts and leaves -- Caffeoylquinic acids -- Cellular antioxidant activity -- Pressurized hot water extraction; Electrophoresis -- Periodicals; Electrophoresis -- Periodicals; 541.372
• DOI: 10.1002/elps.201800063
• Languages: English
• ISSNs: 0173-0835

Abstract

Abstract: Artichoke by‐products are a suitable source of health‐promoting ingredients for the production of dietary supplements and food additives. A pressurized hot water extraction (PHWE) was developed to recover caffeoylquinic acids (CQAs) and flavone glycosides (FLs) from agro‐industrial artichoke by‐products. The main factors influencing PHWE efficiency and CQA isomerization (temperature, numbers of cycles, modifier, and extraction time) were carefully studied and optimized by response surface design. The proposed PHWE procedure provides an exhaustive extraction of CQAs and FLs (recoveries: 93–105% and 90–105%) from artichoke external bracts and leaves of different cultivars ( p > 0.05), without significant formation of artefacts generated by high temperatures. PHWE extracts showed CQA and FL levels (14–37 mg/g and 3–19 mg/g, respectively) comparable to commercial products and marked antioxidative effects (EC50 11–83 μg/mL) by cellular antioxidant activity assay in human hepatocarcinoma HepG2 cells. These results proved that PHWE is an excellent green technique to recover bioactive compounds from artichoke agro‐industrial residues.