Determination of the isoelectric point of shortened glucagon-like peptide-1 by capillary isoelectric focusing with whole column imaging. Issue 5 (3rd September 2018)

• Record Type: Journal Article
• Authors: Fang, Junjian; Liu, Jiexin; Li, Haijing; Cheng, Jianhua; Wu, Shengming; Dong, Fangting
• Is Part Of: Instrumentation science & technology. Volume 46:Issue 5(2018)
• Page Start: 545
• Page End: 554
• Publication Date: 2018-09-03
• Subjects: Capillary isoelectric focusing -- isoelectric point -- shortened glucagon-like peptide-1 -- whole column imaging detection; Scientific apparatus and instruments -- Periodicals; 681.2
• DOI: 10.1080/10739149.2017.1405821
• Languages: English
• ISSNs: 1073-9149

Abstract

ABSTRACT: The isoelectric point of shortened glucagon-like peptide-1, a promising new drug to therapy of type 2 diabetes, was determined using a new generation of capillary isoelectric focusing-whole column imaging. Urea was added as a solubilizer to enhance the stability and repeatability of shortened glucagon-like peptide-1. The isoelectric point of shortened glucagon-like peptide-1 measured by capillary isoelectric focusing-whole column imaging was 4.49, which was similar to the theoretical value of 4.40. Furthermore, the accuracy and repeatability of capillary isoelectric focusing-whole column imaging were investigated. The results showed that this approach provided good accuracy (bias less than 2%) and excellent repeatability (coefficients of variation less than 0.25%). The isoelectric focusing coupled with whole column imaging required only a few minutes for an assay. The study demonstrated that the capillary isoelectric focusing-whole column imaging is useful for the quality control and characterization of peptide and proteins because of its accuracy, repeatability, high performance, and high throughput.