Distinct changes in the proteome profile of endometrial tissues in polycystic ovary syndrome compared with healthy fertile women. Issue 2 (August 2018)

• Record Type: Journal Article
• Authors: Amjadi, Fatemehsadat; Mehdizadeh, Mehdi; Ashrafi, Mahnaz; Nasrabadi, Davood; Taleahmad, Sara; Mirzaei, Mehdi; Gupta, Vivek; Salekdeh, Ghasem Hosseini; Aflatoonian, Reza
• Is Part Of: Reproductive biomedicine online. Volume 37:Issue 2(2018)
• Page Start: 184
• Page End: 200
• Publication Date: 2018-08
• Subjects: Endometrium -- Luteal phase -- PCOS -- Polycystic ovary syndrome -- Proliferative phase -- Proteomics; Human reproductive technology -- Periodicals; Human embryo -- Periodicals; Reproduction -- Periodicals; 616.692
• DOI: 10.1016/j.rbmo.2018.04.043
• Languages: English
• ISSNs: 1472-6483

Abstract

Highlights: Comparative 2D proteomics revealed alterations in the endometrial proteome of PCOS women compared with healthy fertile controls. 70 differentially expressed proteins were identified. Identified proteins are associated with generating endometrial receptivity. Cytoskeleton, apoptosis, oxidative stress and inflammatory response pathways were significantly altered. Abstract: Research question: What is the molecular basis of infertility related to uterine dysfunction in women with polycystic ovary syndrome (PCOS)? Design: In this study, differences in protein expression between PCOS and normal endometrium were identified using a proteomic approach based on two-dimensional electrophoresis (2-DE) coupled with mass spectrometry (MS). The proteome of endometrium were analysed during the proliferative (on day 2 or 3 before ovulation, n = 6) and luteal phases (on day 3–5 after ovulation, n = 6) from healthy women and PCOS patients (12–14 days after spontaneous bleeding, n = 12). The differentially expressed proteins were categorized based on the biological process using the DAVID bioinformatics resources. Results: Over 803 reproducible protein spots were detected on gels, and 150 protein spots showed different intensities between PCOS and normal women during the proliferative and luteal phases. MS analysis detected 70 proteins out of 150 spots. For four of the 70 proteins, 14-3-3 protein, annexin A5, SERPINA1 and cathepsin D, 2-DE results were validated and localized by Western blot and immunohistochemistry, respectively, and their gene expression profiles were confirmed by real-time quantitative PCR. The obtained results corresponded to the proteomic analysis. The differentially expressed proteins identified are known to be involved in apoptosis, oxidative stress, inflammation and the cytoskeleton. Conclusions: The processes related to the differentially expressed proteins play important roles in fecundity and fecundability. The present study may reveal the cause of various endometrial aberrations as a limiting factor for achieving pregnancy in PCOS women.