Biocontrol of Botrytis cinerea and Calonectria gracilis by eucalypts growth promoters Bacillus spp. (August 2018)

• Record Type: Journal Article
• Authors: Paz, Isabel Cristina Padula; Santin, Rita de Cássia Madail; Guimarães, Alexandre Martins; Rosa, Osmar Paulo Pereira da; Quecine, Maria Carolina; Silva, Michele de Cássia Pereira e; Azevedo, João Lúcio; Matsumura, Aida Terezinha Santos
• Is Part Of: Microbial pathogenesis. Volume 121(2018)
• Page Start: 106
• Page End: 109
• Publication Date: 2018-08
• Subjects: Plant diseases -- Biological control -- Eucalypts -- Endophytic bacteria; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2018.05.026
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: The clonal Eucalyptus plants are commonly obtained by vegetative propagation under a protected environment. This system improves the Botrytis cinerea and Calonectria spp infection on the young eucalypts plantings, resulting gray mold and cutting rot respectively. Currently, the unique available control method is based on chemicals. As alternative, novel methods to manage plant diseases, endophytic microorganisms could be an interesting alternative. Thus, we aimed to evaluate endophytic Bacillus isolated from eucalypts as a biocontrol agent against Botrytis cinerea and Calonectria gracilis, important fungal pathogens in the greenhouse, using clonal plantlets of E. urograndis. Eight endophytic strains of Bacillus, previously described as eucalyptus growth promoters, were evaluated in vitro and in vivo against Botrytis cinerea and Calonectria gracilis . The diffusible metabolites assay showed the potential of endophytic Bacillus to decrease the growth of both pathogens. Differences in the susceptibility of the pathogens to bacterial volatile metabolites were observed, B. cinerea showed more susceptible than Calonectria gracilis . In vivo assays, Bacillus amyloliquefaciens EUCB 10 demonstrated better overall reductions in these diseases. Based on the results obtained from the in vitro and in vivo analyses, we suggest that the endophytic B. amyloliquefaciens strain EUCB 10 constitutes a promising biocontrol agent against B. cinerea and Calonectria gracilis . Furthermore, this is the first reporting of B. amyloliquefaciens previously describe as plant growth promoter and also as potential control agent of B. cinerea and Calonectria gracilis to eucalyptus. Highlights: The Bacillus strains were able to inhibit the growth of B. cinerea and C. gracilis. in in vitro e in vivo conditions. The pathogen C. gracilis showed less susceptible to volatile metabolites produced by Bacillus spp. comparing to B. cinerea. Bacillus amyloliquefaciens strain EUCB 10 constitutes a promising biocontrol agent against B. cinerea and C. gracilis. The endophytic environment is a favourable setting to isolate plant growth promoters as well as biological control agents.