Obesity impairs leukocyte‐endothelium cell interactions and oxidative stress in humans. (15th July 2018)

• Record Type: Journal Article
• Authors: López‐Domènech, Sandra; Bañuls, Celia; Díaz‐Morales, Noelia; Escribano‐López, Irene; Morillas, Carlos; Veses, Silvia; Orden, Samuel; Álvarez, Ángeles; Víctor, Víctor M.; Hernández‐Mijares, Antonio; Rocha, Milagros
• Is Part Of: European journal of clinical investigation. Volume 48:Number 8(2018)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2018-07-15
• Subjects: atherogenesis -- humans -- mitochondrial membrane potential -- obesity -- reactive oxygen species; Pathology -- Periodicals; Medical research -- Periodicals; 616.075
• DOI: 10.1111/eci.12985
• Languages: English
• ISSNs: 0014-2972

Abstract

Abstract: Background: To evaluate the relationship between leukocyte‐endothelial cell interactions and oxidative stress parameters in non‐diabetic patients with different grades of obesity. Material and methods: For this cross‐sectional study, 225 subjects were recruited from January 1, 2014 to December 31, 2016 and divided into groups according to BMI (<30 kg/m 2, 30‐40 kg/m 2 and >40 kg/m²). We determined clinical parameters, systemic inflammatory markers, soluble cellular adhesion molecules, leukocyte‐endothelium cell interactions—rolling flux, velocity and adhesion—, oxidative stress parameters—total ROS, total superoxide, glutathione—and mitochondrial membrane potential in leukocytes. Results: We verified that HOMA‐IR and hsCRP increased progressively as obesity developed, whereas A1c, IL6 and TNFα were augmented in the BMI > 40 kg/m² group. The cellular adhesion molecule sP‐selectin was increased in patients with obesity, while sICAM, total ROS, total superoxide and mitochondrial membrane potential were selectively higher in the BMI > 40 kg/m² group. Obesity induced a progressive decrease in rolling velocity and an enhancement of rolling flux and leukocyte adhesion. Conclusion: Our findings reveal that endothelial dysfunction markers are altered in human obesity and are associated with proinflammatory cytokines and increased oxidative stress parameters.