The Proteins from Sika deer antler as potential modulators on cisplatin-induced cytotoxicity in human embryonic kidney 293 cells. Issue 16 (18th August 2018)

• Record Type: Journal Article
• Authors: Yang, Huihai; Li, Wei; Wang, Lulu; He, Xiaofeng; Sun, Hang; Zhang, Jing
• Is Part Of: Natural product research. Volume 32:Issue 16(2018)
• Page Start: 1982
• Page End: 1986
• Publication Date: 2018-08-18
• Subjects: Sika deer antler protein -- cytotoxicity -- cisplatin -- potential modulators -- HEK 293 cell; Human embryonic kidney 293 (HEK 293) -- Sika deer antler protein (SDAPR) -- 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) -- Dimethyl sulfoxide (DMSO) -- Super oxide dismutase (SOD) -- Glutathione (GSH) -- Lactate dehydrogenase (LDH) -- Malondialdehyde (MDA) -- Bicinchoninic acid (BCA) -- Radio-Immunoprecitation (RIPA) -- Phenymethanesulfonyl fluoride (PMSF) -- Tumour necrosis factor alpha (TNF-α) -- Interleukin-6 (IL-6) -- Sodium dodecyl sulfate (SDS) -- The terminal deoxynucleotidyl transferase mediated dUTP nick-end labeling (TUNEL); Natural products -- Periodicals; Natural products -- Research -- Periodicals; Biological Factors -- Periodicals; Biological Products -- Periodicals; 547
• DOI: 10.1080/14786419.2017.1356839
• Languages: English
• ISSNs: 1478-6419

Abstract

Abstract: Our study aimed to investigate the protective role of SDAPR on cisplatin-induced cytotoxicity and its' possible mechanism in HEK293 cells. Cell viability was measured by MTT assay. Oxidative stress (SOD, GSH, LDH and MDA), inflammatory factors (TNF-α and IL-6) and apoptosis-related proteins (caspase-3, Bax, Bcl-2) expression were measured. The apoptotic cells were observed by TUNEL staining. Our study results indicated that non-cytotoxic levels of SDAPR significantly increased viability rate (LD50 value of cisplatin is 20 μM), which improved antioxidant defence, attenuated apoptosis by decreasing expression levels of cleaved-caspase-3 and Bax, increasing Bcl-2 expression and inhibiting apoptotic positive cells in HEK 293 cells. In addition, SDAPR treatment markedly inhibited the levels of TNF-α and IL-6. In conclusion, Sika deer antler protein, a potential modulator, could alleviate cisplatin-induced cytotoxicity in HEK 293 cells. Abstract :