Immunomagnetic bead-based biotin-streptavidin system for highly efficient detection of aflatoxin B1 in agricultural products. Issue 46 (19th July 2018)

• Record Type: Journal Article
• Authors: Li, Ming; Zhang, Yuanyuan; Zhao, Rujin; Liu, Zhenjiang; Hong, Xia; Cui, Yin; Xue, Yonglai; Du, Daolin
• Is Part Of: RSC advances. Volume 8:Issue 46(2018)
• Page Start: 26029
• Page End: 26035
• Publication Date: 2018-07-19
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c8ra04460k
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : A strategy of immunomagnetic beads coupled with biotin-streptavidin system (BAS-IMB) for detecting AFB1 was suggested and applied in agricultural products. Abstract : The potential homogeneous assay employing immunomagnetic beads (IMB) has been receiving attention as a screening tool in food-safety control; the method is simple, efficient, and does not require long incubation times or complex separation steps. In this study, a homogeneous immunoassay has been successfully developed and applied in the determination of aflatoxin B1 (AFB1 ) contamination in agricultural products by coupling IMB and the biotin-streptavidin (BSA) (BSA-IMB) system. Under optimal conditions, the limit of detection (LOD, IC10 ), half-maximal inhibition concentration (IC50 ) and detection range (IC20 –IC80 ) of BSA-IMB are 0.00579, 0.573 and 0.0183–17.9 ng mL −1, respectively, for AFB1 . The detection of AFB1 by BSA-IMB can be achieved in 40 min (ELISA needs at least 180 min). The cross-reactivities of BSA-IMB with its analogues are negligible (<3.82%); these results indicate high selectivity. The spiked recoveries are in the range from 89.6 to 118.2% with relative standard deviations (RSDs) of 3.4 to 13.2% for AFB1 in agricultural product samples. Furthermore, the results of BSA-IMB for authentic samples show reliability and high correlation of 0.9928 with an HPLC-fluorescence detector. The proposed BSA-IMB system is demonstrated to be a satisfactory tool for homogeneous, efficient, sensitive, and alternative detection of AFB1 in a wide detection range for agricultural product samples.