A supramolecular host–guest complex for heparin binding and sensing. Issue 29 (11th July 2018)

• Record Type: Journal Article
• Authors: Välimäki, Salla; Beyeh, Ngong Kodiah; Linko, Veikko; Ras, Robin H. A.; Kostiainen, Mauri A.
• Is Part Of: Nanoscale. Volume 10:Issue 29(2018)
• Page Start: 14022
• Page End: 14030
• Publication Date: 2018-07-11
• Subjects: Nanoscience -- Periodicals; Nanotechnology -- Periodicals; 620.505
• DOI: 10.1039/c8nr03132k
• Languages: English
• ISSNs: 2040-3364

Abstract

Abstract : A cationic pillararene-dye host–guest complex capable of heparin binding and sensing is presented. Abstract : Heparin is an anionic polysaccharide widely used in clinics as an anticoagulant. However, heparin usage requires an antidote and sensors for safe operation during and after surgeries. In this study, a host–guest complex capable of selective heparin binding and sensing is presented. Heparin binding affinity was studied in solution with a variety of polycationic macrocyclic hosts, a pillar[5]arene and multiple resorcin[4]arenes, by dynamic light scattering, dye displacement assay, isothermal titration calorimetry, and anti-Xa assay. The measurements reveal the significant importance of multivalency in electrostatic host–heparin binding in competitive, application-relevant media. Additionally, to monitor the heparin concentration, a host–guest indicator displacement assay was performed by following the free and bound state of the methyl orange dye in UV-Vis spectroscopic experiments. Furthermore, this colorimetric sensing based on the tertiary host–guest–heparin supramolecular assembly was utilized in the construction of a calibration curve in a range of blood plasma concentrations.