Controlling the orientation of a cell-synthesized extracellular matrix by using engineered gelatin-based building blocks. (21st June 2018)

• Record Type: Journal Article
• Authors: Pennacchio, Fabrizio A.; Casale, Costantino; Urciuolo, Francesco; Imparato, Giorgia; Vecchione, Raffaele; Netti, Paolo A.
• Is Part Of: Biomaterials science. Volume 6:Number 8(2018)
• Page Start: 2084
• Page End: 2091
• Publication Date: 2018-06-21
• Subjects: Biomedical materials -- Periodicals; 610.28
• DOI: 10.1039/c7bm01093a
• Languages: English
• ISSNs: 2047-4830

Abstract

Abstract : Surface micropatterned gelatin building blocks clearly increment the alignment degree of collagen-based microtissues synthesized by human dermal fibroblasts. Abstract : In tissue engineering there is growing interest in fabricating highly engineered platforms designed to instruct cells towards the synthesis of tissues that reproduce their natural counterpart. In this context, a fundamental factor to take into account is the control over the final tissue orientation, especially for what concerns the replication of load-bearing tissues whose functions are strictly related to their microstructural organization. Starting from this point, in this work we have engineered a gelatin-based hydrogel in order to be patterned by 2-photon polymerization (2PP) lithography for the fabrication of instructive free standing building blocks designed to produce anisotropic collagen-based μtissues. Biological results clearly highlighted the strong relationship between μtissue orientation and such topographies, which resulted in a crucial element in the production of highly anisotropic μtissues.