A graphene-based fluorescent nanoprobe for simultaneous monitoring of miRNA and mRNA in living cells. Issue 29 (16th July 2018)

• Record Type: Journal Article
• Authors: Pan, Wei; Liu, Bo; Gao, Xiaonan; Yu, Zhengze; Liu, Xiaohan; Li, Na; Tang, Bo
• Is Part Of: Nanoscale. Volume 10:Issue 29(2018)
• Page Start: 14264
• Page End: 14271
• Publication Date: 2018-07-16
• Subjects: Nanoscience -- Periodicals; Nanotechnology -- Periodicals; 620.505
• DOI: 10.1039/c8nr04106g
• Languages: English
• ISSNs: 2040-3364

Abstract

Abstract : A graphene-based fluorescent nanoprobe was developed to monitor miRNA and mRNA simultaneously in living cells. Abstract : Since microRNA-21 (miR-21) and protein programmed cell death 4 (PDCD4) are implicated in tumor progression, simultaneous monitoring of the dynamic variation of miR-21 and PDCD4 mRNA in living cells is of great interest for understanding their relationship in the pathology of the disease. Herein, a versatile nanoprobe based on graphene oxide (GO) and DNA was prepared which can simultaneously monitor and visualize miR-21 and PDCD4 mRNA in living cells. In vitro experiments demonstrate that the nanoprobe exhibits outstanding selectivity and high sensitivity towards miR-21 and PDCD4 mRNA. Moreover, by exploiting the DNA/GO sensing platform, simultaneous imaging of miR-21 and PDCD4 mRNA in MCF-7 human breast cancer cells has been realized. More significantly, an inverse correlation between miR-21 and PDCD4 mRNA was verified by fluorescence confocal imaging and RT-PCR results, indicating the distinguishing ability of the nanoprobe at various expression levels of RNAs. The current strategy provides a promising tool to implement the detection of miR-21 and PDCD4 mRNA in cancer cells and can be expected to be applied for other biomolecular sensing.