Hydrogen sulfide oxidation under anoxic conditions by a nitrate-reducing, sulfide-oxidizing bacterium isolated from the Mae Um Long Luang hot spring, Thailand. (October 2017)

• Record Type: Journal Article
• Authors: Watsuntorn, Wannapawn; Ruangchainikom, Chalermchai; Rene, Eldon R.; Lens, Piet N.L.; Chulalaksananukul, Warawut
• Is Part Of: International biodeterioration & biodegradation. Volume 124(2017)
• Page Start: 196
• Page End: 205
• Publication Date: 2017-10
• Subjects: Anoxic biodegradation -- Hydrogen sulfide removal -- NR-SOB -- Kinetics; Biodegradation -- Periodicals; Bioremediation -- Periodicals; Biodegradation -- Periodicals; Biodégradation -- Périodiques; Biorestauration -- Périodiques; Electronic journals; 620.11223
• DOI: 10.1016/j.ibiod.2017.06.013
• Languages: English
• ISSNs: 0964-8305

Abstract

Abstract: Biological removal of hydrogen sulfide (H2 S) has been widely practiced across the world because it overcomes the drawbacks associated with physico-chemical processes. In this study, the role of a novel MAL 1HM19 strain of a nitrate-reducing, sulfide-oxidizing bacterium (NR-SOB) isolated from the Mae Um Long Luang hot spring (Thailand) was investigated in batch systems for H2 S removal under different initial N-NO3 − concentrations (60, 120 and 240 mg N-NO3 − /L) at 35 °C. The strain MAL 1HM19 was capable of removing 100% of the gas-phase H2 S within 10 h of incubation. In the liquid-phase, the final products depended on the initial N-NO3 − concentration: at 120 and 240 mg N-NO3 − /L, 15% and 18% SO4 2− formation was detected, while no SO4 2− was detected at an initial N-NO3 − concentration of 60 mg N-NO3 − /L. The identification of strain MAL 1HM19, based on the 16S rDNA nucleotide sequence, revealed it was closely related to Paracoccus sp. (99.93% similarity). The soxB gene was present in strain MAL 1HM19. In addition, strain MAL 1HM19 showed a good capacity to grow at several NaCl concentrations (0.03–7% w/v ), at temperatures varying between 20 and 50 °C and within the pH range of 7.0–9.0. Graphical abstract: Highlights: 100% H2 S was removed completely within 10 h by strain MAL 1HM19. The end-products via H2 S oxidation relied on the initial nitrate concentration. Strain MAL 1HM19 is closely related to Paracoccus sp. (99.93% similarity). soxB gene was detected in strain MAL 1HM19. MAL 1HM19 tolerated high NaCl concentrations, pH 7.0–9.0 and temperatures 20–50 °C.