Characterisation of in vitro gastrointestinal digests from low fat caprine kefir enriched with inulin. (December 2017)

• Record Type: Journal Article
• Authors: Simsek, Sebnem; Sánchez-Rivera, Laura; El, Sedef Nehir; Karakaya, Sibel; Recio, Isidra
• Is Part Of: International dairy journal. Volume 75(2017)
• Page Start: 68
• Page End: 74
• Publication Date: 2017-12
• Subjects: Dairying -- Periodicals; Industrie laitière -- Périodiques; Electronic journals; 338.1762142
• DOI: 10.1016/j.idairyj.2017.07.004
• Languages: English
• ISSNs: 0958-6946

Abstract

Abstract: In vitro gastrointestinal digests from low fat caprines' milk kefir were characterised. The impact of the addition of different types of inulin (native, short-chain and long-chain) as a fat replacer on the subsequent release of peptides during digestion was also studied. A total of 52 peptides were identified, mainly from β-casein, following digestion. The gastrointestinal digests possessed angiotensin-converting enzyme (ACE)-inhibitory activity with IC50 values ranging from 190.71 ± 2.91 to 364.68 ± 31.27 μg protein mL −1 . Among the identified peptides, four of them have been previously described as ACE-inhibitors (LHLPLP, HLPLP, DKIHP, MAIPPK), and the presence of these sequences can explain the moderate ACE-inhibitory activity found in the samples. Rheological analysis revealed that all manufactured kefirs exhibited shear-thinning behaviour. No significant effect of viscosity on protein degradation during simulated gastrointestinal digestion was found under the working conditions.