Effects of esculetin on lipopolysaccharide (LPS)-induced acute lung injury via regulation of RhoA/Rho Kinase/NF-кB pathways in vivo and in vitro. (2nd December 2015)

• Record Type: Journal Article
• Authors: Chen, Tong; Guo, Qianqian; Wang, Huimin; Zhang, Huixin; Wang, Ciman; Zhang, Ping; Meng, Shanshan; Li, Yunman; Ji, Hui; Yan, Tianhua
• Is Part Of: Free radical research. Volume 49:Number 12(2015:Dec.)
• Page Start: 1459
• Page End: 1468
• Publication Date: 2015-12-02
• Subjects: Esculetin -- lipopolysaccharide (LPS) -- acute lung injury -- lung epithelial A549 -- RhoA/Rho kinase/NF-кB; Free radicals (Chemistry) -- Periodicals; Antioxidants -- Periodicals; Vitamin C -- Periodicals; Vitamin E -- Periodicals; 541.224
• DOI: 10.3109/10715762.2015.1087643
• Languages: English
• ISSNs: 1071-5762

Abstract

Abstract : The purpose of the present study was to investigate the protective effect of esculetin (ES) in lipopolysaccharide (LPS)-induced acute lung injury (ALI) and the lung epithelial A549 cells. Mice were intragastrically administered with ES (20 and 40 mg/kg) 1 h prior to LPS challenge. ES pretreatment at doses of 20 and 40 mg/kg effectively attenuated LPS-induced lung histopathological change, myeloperoxidase or MPO activity, inflammatory cells infiltration, pulmonary wet-to-dry weight ratio, and the generation of pro-inflammatory cytokines including tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6) in vivo and in vitro. Furthermore, we demonstrated that ES blocked the activation of NF-кB and RhoA/Rho kinase pathways in LPS-induced mice and A549 cells. The results suggested that ES exhibited protective effect on ALI and might attribute partly to the inhibition of NF-кB and RhoA/Rho kinase pathways in vivo and in vitro.