Infection of a nucleopolyhedrovirus to Neodiprion zhejiangenis Zhou & Xiao (Hymenoptera: Diprionidae). Issue 8 (3rd August 2018)

• Record Type: Journal Article
• Authors: Wang, Qinghua; Li, Enjie; Li, Na; Wang, Yuzhu; Zhang, Zhilin; Zhang, Yongan
• Is Part Of: Biocontrol science and technology. Volume 28:Issue 8(2018)
• Page Start: 761
• Page End: 771
• Publication Date: 2018-08-03
• Subjects: Neodiprion zhejiangensis Zhou & Xiao -- nucleopolyhedrovirus -- viral susceptibility -- lethal time -- virus effects on survivors; Pests -- Biological control -- Periodicals; Weeds -- Biological control -- Periodicals; Pesticides -- Environmental aspects -- Periodicals; Plants, Protection of -- Periodicals; Plants -- Biological control -- Periodicals; Agricultural pests -- Control -- Periodicals; Pest Control, Biological -- Periodicals; 632.96
• DOI: 10.1080/09583157.2018.1493089
• Languages: English
• ISSNs: 0958-3157

Abstract

ABSTRACT: Neodiprion zhejiangenis Zhou & Xiao 1981 (Hymenoptera: Diprionidae) nuclear polyhedrosis virus (NezhNPV) is a biocontrol factor with high development potential and application prospects. To study the insecticidal activity of NezhNPV against the larvae of N. zhejiangenis, the susceptibility of larvae at different instars to different NezhNPV concentrations was determined based on laboratory bioactivity. At 2.0 × 10 7 polyhedron inclusion body (PIB)/mL, the median lethal time required to kill 50% of the instar 2 and 3 larvae was approximately 5 d, whereas the median lethal time of the instar 4 and 5 larvae was 8–9 d. Interestingly, at the NezhNPV concentrations of 2.0 × 10 3 and 2.0 × 10 4 PIB/mL, the feeding ability of the surviving larvae was decreased by 57.4% and 76.4%, respectively, compared with the controls; the pupal weight at both concentrations was decreased by approximately 27%, and the adult emergence rate was decreased by 27.4% and 50.9%, respectively, with a significantly higher proportion of males than of females. The results showed that younger instar larvae of N. zhejiangenis were more susceptible to NezhNPV infection than older instar larvae. The larval mortality rate was dependent on the larval instar and NezhNPV concentration.