Development and application of a HPLC-MS/MS method for quantitation of fucosylated chondroitin sulfate and fucoidan in sea cucumbers. (August 2018)

• Record Type: Journal Article
• Authors: Zhu, Zhenjun; Zhu, Beiwei; Ai, Chunqing; Lu, Jiaojiao; Wu, Sufeng; Liu, Yili; Wang, Linlin; Yang, Jingfeng; Song, Shuang; Liu, Xiaoling
• Is Part Of: Carbohydrate research. Volume 466(2018)
• Page Start: 11
• Page End: 17
• Publication Date: 2018-08
• Subjects: Sea cucumber polysaccharides -- Fucosylated chondroitin sulfate -- Fucoidan -- Acid hydrolysis -- HPLC–MS/MS; Carbohydrates -- Periodicals; Chemistry, Organic -- Periodicals; Biochemistry -- Periodicals; Carbohydrates -- Periodicals; Chimie organique -- Périodiques; Glucides -- Périodiques; Biochemistry; Carbohydrates; Chemistry, Organic; Periodicals; Electronic journals; 507.78
• DOI: 10.1016/j.carres.2018.07.001
• Languages: English
• ISSNs: 0008-6215

Abstract

Abstract: Fucosylated chondroitin sulfate (FCS) and fucoidan (FUC) are two main bioactive polysaccharides in sea cucumbers. A novel method for quantitation of FCS and FUC was developed by detecting chondroitin disaccharide and fucose produced through acid hydrolysis using HPLC-MS/MS. The present method showed satisfactory performance for both saccharides. It was applied to assay sea cucumbers ( Stichopus japonicus ) reared in pond grow-out or bottom sowing, and the results were compared with those obtained by traditional HPLC method and 1, 9-dimethylmethylene blue test, which could only provide the total sea cucumber polysaccharide (SCP) contents. No difference of total SCP content was observed between sea cucumbers reared through different ways, while a higher ratio of FCS to FUC of sea cucumbers of pond grow-out was revealed by the present method. Thus, this novel method is potential to quantify the two polysaccharides and could be a powerful tool for quality evaluation of sea cucumbers. Graphical abstract: Highlights: A method for quantitation of two polysaccharides in sea cucumbers was developed. The method showed satisfactory performance for both polysaccharides. The method revealed differences between sea cucumbers cultured differently.