Beneficial effect of baicalin on insulin sensitivity in adipocytes of diet-induced obese mice. (May 2018)

• Record Type: Journal Article
• Authors: Fang, Penghua; Yu, Mei; Min, Wen; Han, Shiyu; Shi, Mingyi; Zhang, Zhenwen; Bo, Ping
• Is Part Of: Diabetes research and clinical practice. Volume 139(2018)
• Page Start: 262
• Page End: 271
• Publication Date: 2018-05
• Subjects: Baicalin -- GLUT4 -- Insulin sensitivity -- Adipocytes; Diabetes -- Periodicals; Diabetes Mellitus -- Periodicals; 616.462
• DOI: 10.1016/j.diabres.2018.03.007
• Languages: English
• ISSNs: 0168-8227

Abstract

Highlights: Administration of baicalin increased glucose uptake in adipocytes. Administration of baicalin increased pAKT and PGC-1α levels in adipocytes. Administration of baicalin decreased p-p38 MAPK and pERK levels in adipocytes. Administration of baicalin improved GLUT4 expression and translocation in adipocytes. Abstract: Aims: Although baicalin has been shown to increase glucose uptake and insulin sensitivity in skeletal muscle of mice, there is no literature available about the effect of baicalin on insulin sensitivity in adipocytes of diet-induced obese mice. Methods: In the present study, diet-induced obese mice were given 50 mg/kg baicalin intraperitoneally (i.p.) once a day for 21 days, and 3T3-L1 cells were treated with 100, 200, 400 μM baicalin for 3 h. Then insulin resistance indexes and insulin signal protein levels were examined to elucidate whether baicalin increased glucose uptake and GLUT4 translocation in adipocytes of diet-induced obese mice. Results: The present findings showed that administration of baicalin decreased food intake, body weight, HOMA-IR and p-p38 MAPK and pERK levels, but enhanced pAKT and PGC-1α contents, as well as GLUT4 mRNA, PGC-1α mRNA expression in adipocytes, and reversed high fat diet-induced glucose intolerance, hyperglycemia and insulin resistance in diet-induced obese mice. Moreover, baicalin treatment increased GLUT4 concentration in plasma membranes of adipocytes. Conclusions: These data demonstrated that baicalin accelerated GLUT4 translocation from intracellular membrane compartments to plasma membranes in adipocytes. Baicalin plays a significant role in elevation of glucose uptake and insulin sensitivity to promote glucose clearance.