Expression of Cathepsin F in response to bacterial challenges in Yesso scallop Patinopecten yessoensis. Issue 80 (September 2018)

• Record Type: Journal Article
• Authors: Guo, Haobing; Li, Yangping; Zhang, Meiwei; Li, Ruojiao; Li, Wanru; Lou, Jiarun; Bao, Zhenmin; Wang, Yangfan
• Is Part Of: Fish & shellfish immunology. Issue 80(2018)
• Page Start: 141
• Page End: 147
• Publication Date: 2018-09
• Subjects: Cathepsin F -- Patinopecten yessoensis -- Innate immune response -- Gram-positive and Gram-negative challenges; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2018.06.005
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Cathepsin F is a unique papain cysteine proteinase with highly conserved structures: catalytic triad and a cystatin domain contained in the elongated N-terminal pro-region. It has been reported that cathepsin F is associated with the establishment of innate immune in several vertebrate including fish in aquaculture, but not known in bivalves. In this study, we firstly identified and characterized cathepsin F in the Yesso scallop ( Patinopecten yessoensis ). The protein structural and phylogenetic analyses were then conducted to determine its identity and evolutionary position. We've also investigated the expression levels of cathepsin F gene at different embryonic developmental stages, in healthy adult tissues and especially in the hemocytes and hepatopancreas after Gram-positive ( Micrococcus luteus ) and negative ( Vibrio anguillarum ) challenges using quantitative real-time PCR (qPCR). Cathepsin F was significantly up-regulated 3 h after infection of V. anguillarum in hemocytes, suggesting its participation in immune response. Our findings have provided strong evidence that cathepsin F may be a good target for enhancing the immune activity in Yesso scallop. Highlights: A cathepsin F gene was identified in the Yesso scallop. Phylogenic analysis implied cathepsin F proteins were analogous in invertebrate and evolutionarily conserved. Spatiotemporal expression of cathepsin F indicated the existence of maternal immune transfer. Bacteria challenges suggested the involvement of cathepsin F in immune response.