Multi-generational xenoestrogenic effects of Perfluoroalkyl acids (PFAAs) mixture on Oryzias latipes using a flow-through exposure system. (February 2017)

• Record Type: Journal Article
• Authors: Lee, Jin Wuk; Lee, Jae-Woo; Shin, Yu-Jin; Kim, Ji-Eun; Ryu, Tae-Kwon; Ryu, Jisung; Lee, Jaean; Kim, Pilje; Choi, Kyunghee; Park, Kyunghwa
• Is Part Of: Chemosphere. Volume 169(2017)
• Page Start: 212
• Page End: 223
• Publication Date: 2017-02
• Subjects: Perfluorooctane sulfonate (PFOS) -- Perfluroroctanoic acid (PFOA) -- Perfluorobutane sulfonate (PFBS) -- Perfluorononanoic acid (PFNA) -- Oryzias latipes -- Multi-generational estrogenic effects; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2016.11.035
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: To elucidate the multi-generational estrogenic potential of Perfluoroalkyl acids (PFAAs) mixture, vitellogenin (VTG) expression, growth indices, histological alteration, fecundity, hatching rate, larval survival rate, and sex ratio of Japanese medaka ( Oryzias latipes ) were investigated by exposing the fish to a mixture of perfluorooctane sulfonate (PFOS), perfluroroctanoic acid (PFOA), perfluorobutane sulfonate (PFBS), and perfluorononanoic acid (PFNA) for three generations (238 days). Mixture composition is in the ratio of 1:1:1:1. In addition, whole body burden for each PFAA was analyzed. According to the results, concentrated levels of the PFAAs in both F1 and F2 generation O. latipes were ordered PFOS > PFNA > PFOA > PFBS at both low concentration (0.5 μg/L) and high concentration (5 μg/L), whereas a significant difference in whole body burden based on sex or generation was not detected. Significant induction of VTG expression in F2 and the decline of the gonad somatic index (GSI) in F1 were observed following PFAAs mixture exposure ( p < 0.05, one-way ANOVA). Furthermore, suppression level of reproduction rate relative to the control increased as generation was transferred to the next in response to PFAAs mixture or 17 β-estradiol exposure, with the inhibition of hatchability observed in the F1 generation. The PFAA high concentration caused significant alteration of F1 generation sex ratio, suggesting the adverse effect of PFAA in population level (Chi-square test, P > 0.05). Overall, this study demonstrated that PFAA mixture could have the potential of multi-generational endocrine disruptors in O. latipes . Highlights: In all PFAAs, PFOS showed the highest cumulative potential in Oryzias latipes. PFAAs caused significant increase of vitellogenin expression only in last generation, F2. Fecundity was suppressed by PFAAs exposure as generation goes from F0 to F2. PFAAs caused sex ratio disturbance relative to control.