MicroRNA-27b inhibits cell proliferation in oral squamous cell carcinoma by targeting FZD7 and Wnt signaling pathway. (November 2017)

• Record Type: Journal Article
• Authors: Liu, Bingyao; Chen, Wei; Cao, Gang; Dong, Zhen; Xu, Jinke; Luo, Tingyuan; Zhang, Senlin
• Is Part Of: Archives of oral biology. Volume 83(2017)
• Page Start: 92
• Page End: 96
• Publication Date: 2017-11
• Subjects: Oral squamous cell carcinoma -- miR-27b -- Cell proliferation -- FZD7 -- Wnt signaling pathway; Mouth -- Periodicals; Mouth -- Diseases -- Periodicals; Dentistry -- Periodicals; Electronic journals; 617.6005
• DOI: 10.1016/j.archoralbio.2017.07.009
• Languages: English
• ISSNs: 0003-9969

Abstract

Highlights: miR-27b was down-regulated in OSCC cell lines. Overexpressed miR-27b inhibited OSCC cell proliferation by directly targeting FZD7. FZD7 expression was inversely correlated with miR-27b expression in OSCC cell lines. Abstract: This study intended to investigate the role of microRNA-27b (miR-27b) in proliferation of oral squamous cell carcinoma (OSCC) cells and to explore the potential molecular mechanism. Cell proliferation was detected by MTT assay. The expression levels of miR-27b, Frizzled7 ( FZD7 ), cyclin D1 and c-myc were detected by quantitative real time polymerase chain reaction (qRT-PCR). The protein expression level of FZD7 was detected by western blot analysis. The relationship between miR-27b and FZD7, and the activity of Wnt signaling pathway were determined using luciferase reporter assay. The miR-27b expression in OSCC cell lines was significantly decreased compared with control. Overexpression of miR-27b remarkably inhibited OSCC cell proliferation. Additionally, miR-27b could target and inhibit FZD7 expression and decrease the activity of Wnt signaling pathway.miR-27b could inhibit OSCC cell proliferation through inhibiting FZD7 and FZD7-mediated Wnt signaling pathway.