Regulatory motifs on ISWI chromatin remodelers: molecular mechanisms and kinetic proofreading. (7th January 2015)

• Record Type: Journal Article
• Authors: Brysbaert, Guillaume; Lensink, Marc F; Blossey, Ralf
• Is Part Of: Journal of physics. Volume 27:Number 6(2015)
• Publication Date: 2015-01-07
• Subjects: chromatin -- chromatin remodeling -- kinetic proofreading; Condensed matter -- Periodicals; Matière condensée -- Périodiques; Vaste stoffen; Vloeistoffen; Natuurkunde; Electronic journals; Computer network resources; 530.4105
• DOI: 10.1088/0953-8984/27/6/064108
• Languages: English
• ISSNs: 0953-8984

Abstract

Abstract: Recently, kinetic proofreading scenarios have been proposed for the regulation of chromatin remodeling, first on purely theoretical grounds (Blossey and Schiessel 2008 HFSP J. 2 167–70 ) and deduced from experiments on the ISWI/ACF system (Narlikar 2010 Curr. Opin. Chem. Biol. 14 660 ). In the kinetic proofreading scenario of chromatin remodeling, the combination of the recognition of a histone tail state and ATP-hydrolysis in the remodeler motor act together to select (i.e. proofread) a nucleosomal substrate. ISWI remodelers have recently been shown to have an additional level of regulation as they contain auto-inhibitory motifs which need to be inactivated through an interaction with the nucleosome. In this paper we show that the auto-regulatory effect enhances substrate recognition in kinetic proofreading. We further report some suggestive additional insights into the molecular mechanism underlying ISWI-autoregulation.