Nanowire Arrays as Force Sensors with Super‐Resolved Localization Position Detection: Application to Optical Measurement of Bacterial Adhesion Forces. Issue 7 (12th June 2018)

• Record Type: Journal Article
• Authors: da Silva, Aldeliane M.; Sahoo, Prasana K.; Cavalli, Alessandro; de Souza, Alessandra A.; Bakkers, Erik P. A. M.; Cesar, Carlos L.; Janissen, Richard; Cotta, Monica A.
• Is Part Of: Small methods. Volume 2:Issue 7(2018)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2018-06-12
• Subjects: biofilms -- cell adhesion forces -- confocal microscopy -- nanowires -- Xylella fastidiosa; Nanotechnology -- Methodology -- Periodicals; Nanotechnology -- Periodicals; Periodicals; 620.5028
• DOI: 10.1002/smtd.201700411
• Languages: English
• ISSNs: 2366-9608

Abstract

Abstract: The design and application of indium phosphide (InP) nanowire arrays to acquire Xylella fastidiosa bacterial cell vector force maps are discussed. The nanowire deflections are measured with subdiffraction localization confocal laser scanning microscopy (CLSM). The nanowire mechanical stability in air and liquid media as well as methods to average out thermally induced oscillations are investigated. The accuracy of center determination of the CLSM reflected laser intensity profile at nanowire apex is studied using Gaussian fitting and localization microscopy techniques. These results show that the method is reliable for measuring nanowire displacements above ≈25 nm. Corresponding force ranges probed by this method can be customized depending on nanowire geometry and array configuration. The method is applied to explore X. fastidiosa cell adhesion forces on the InP nanowire surface, and in situ probes the effect of N ‐acetylcysteine on adhered cells. Future perspectives for application of this method in microbiology studies are also outlined. Abstract : Indium phosphide (InP) nanowire arrays are used as bacterial adhesion force sensors, with nanowire deflections measured with subdiffraction localization confocal laser scanning microscopy, rendering minimized thermally induced oscillations. Xylella fastidiosa cell adhesion forces on the InP nanowire surface are probed in situ to illustrate the disrupting effect of N ‐acetylcysteine on single cell and small cluster surface adhesion.