Targeted in situ metatranscriptomics for selected taxa from mesophilic and thermophilic biogas plants. Issue 4 (4th December 2017)

• Record Type: Journal Article
• Authors: Stolze, Yvonne; Bremges, Andreas; Maus, Irena; Pühler, Alfred; Sczyrba, Alexander; Schlüter, Andreas
• Is Part Of: Microbial biotechnology. Volume 11:Issue 4(2018:Jul.)
• Page Start: 667
• Page End: 679
• Publication Date: 2017-12-04
• Subjects: Microbial biotechnology -- Periodicals; Biotechnology; Microbiology; 660.62
• DOI: 10.1111/1751-7915.12982
• Languages: English
• ISSNs: 1751-7915

Abstract

Summary: Biogas production is performed anaerobically by complex microbial communities with key species driving the process. Hence, analyses of their in situ activities are crucial to understand the process. In a previous study, metagenome sequencing and subsequent genome binning for different production‐scale biogas plants (BGPs) resulted in four genome bins of special interest, assigned to the phyla Thermotogae, Fusobacteria, Spirochaetes and Cloacimonetes, respectively, that were genetically analysed. In this study, metatranscriptome sequencing of the same BGP samples was conducted, enabling in situ transcriptional activity determination of these genome bins. For this, mapping of metatranscriptome reads on genome bin sequences was performed providing transcripts per million (TPM) values for each gene. This approach revealed an active sugar‐based metabolism of the Thermotogae and Spirochaetes bins and an active amino acid‐based metabolism of the Fusobacteria and Cloacimonetes bins. The data also hint at syntrophic associations of the four corresponding species with methanogenic Archaea . Abstract : In this study, the applied genome bin‐based metatranscriptomics approach enabled insights into the in situ transcriptional activity of four mostly non‐cultivated and novel species represented by the genome bins in their respective habitats and therefore complements analogous analyses for isolates from biogas communities.