Cytocompatibility of Biodentine and iRoot FS with human periodontal ligament cells: an in vitro study. (6th February 2018)

• Record Type: Journal Article
• Authors: Luo, T.; Liu, J.; Sun, Y.; Shen, Y.; Zou, L.
• Is Part Of: International endontic journal. Volume 51:Number 7(2018)
• Page Start: 779
• Page End: 788
• Publication Date: 2018-02-06
• Subjects: biodentine -- cell differentiation -- cell proliferation -- human periodontal ligament cells -- iRoot Fast Set root repair material; Endodontics -- Periodicals; 617.6342
• DOI: 10.1111/iej.12889
• Languages: English
• ISSNs: 0143-2885

Abstract

Abstract: Aim: To evaluate the cytocompatibility of Biodentine and iRoot FS with human periodontal ligament cells (hPDLCs). Methodology: Human periodontal ligament cells were characterized by flow cytometry and immunocytochemical analysis. Human periodontal ligament cell adhesion was assessed by scanning electron microscopy at day 3; proliferation by live/dead assay at days 1, 3 and 7; and osteogenic differentiation by alkaline phosphatase (ALP) activity staining, ALP quantification analysis and qRT‐PCR at days 7 and 14. Data were analysed withanova and independent sample t‐ tests with SPSS 21.0. Results: Both iRoot FS and Biodentine increased the adhesion of hPDLCs at day 3. Compared to Biodentine, iRoot FS positively increased hPDLC proliferation on days 3 ( P = 0.03) and 7 ( P = 0.00). Osteogenic marker ALP was observed consistently in all samples, with iRoot FS having significantly higher ALP activity at day 14 ( P = 0.00). Compared with Biodentine, iRoot FS significantly increased the mRNA level of ALP, COL1 and Runx2, and OCN increased only on day 14 ( P < 0.05). Conclusions: iRoot FS had a positive effect on the adhesion, proliferation and biomineralization of hPDLCs compared with Biodentine.