Comparison of open and closed abdomen techniques for the delivery of intraperitoneal pemetrexed using a murine model. Issue 6 (22nd January 2018)

• Record Type: Journal Article
• Authors: Badrudin, David; Sideris, Lucas; Perrault‐Mercier, Camille; Hubert, Julien; Leblond, François A.; Dubé, Pierre
• Is Part Of: Journal of surgical oncology. Volume 117:Issue 6(2018)
• Page Start: 1318
• Page End: 1322
• Publication Date: 2018-01-22
• Subjects: experimental study -- HIPEC -- pemetrexed; Cancer -- Surgery -- Periodicals; Neoplasms -- Periodicals; 616
• DOI: 10.1002/jso.24960
• Languages: English
• ISSNs: 0022-4790

Abstract

Abstract : Background and Objectives: Pemetrexed is an appealing agent to use for cytoreductive surgery with hyperthermic intraperitoneal chemotherapy (HIPEC). However, the optimal method of pemetrexed delivery still remains undefined. Using a murine model, we compared the use of open and closed abdomen techniques on the absorption of intraperitoneal (IP) pemetrexed in different compartments. Methods: Eleven Sprague‐Dawley rats were submitted to a fixed dose of IP pemetrexed (1000 mg/m 2 ) at a perfusion temperature of 40°C during 25 min according to two techniques: open and closed. At the end of perfusion, samples in different compartments were harvested and the concentrations of pemetrexed were measured by high performance liquid chromatography. Results: Absorption of IP pemetrexed in portal and systemic blood was significantly higher using the open compared to the closed abdomen technique (93.17 vs 52.50 µg/mL, P < 0.001) and (76.26 vs 51.65 µg/mL, P < 0.001), respectively. No difference was found between the two techniques on the peritoneal tissue concentration of pemetrexed (18.07 vs 19.17 µg/g, P = 0.51). Conclusion: Peritoneal absorption of pemetrexed is not modified by the use of either technique. However, systemic concentrations of pemetrexed increased using the open technique, suggesting it could increase systemic toxicity.