MiR‐146a regulates PM1‐induced inflammation via NF‐κB signaling pathway in BEAS‐2B cells. Issue 7 (18th April 2018)

• Record Type: Journal Article
• Authors: Liu, Limin; Wan, Chong; Zhang, Wei; Guan, Longfei; Tian, Guoxiong; Zhang, Fang; Ding, Wenjun
• Is Part Of: Environmental toxicology. Volume 33:Issue 7(2018)
• Page Start: 743
• Page End: 751
• Publication Date: 2018-04-18
• Subjects: BEAS‐2B cells -- inflammation -- miR‐146a -- NF‐κB -- PM1; Water quality bioassay -- Periodicals; Water -- Pollution -- Toxicology -- Periodicals; Microbiological assay -- Periodicals; Toxicity testing -- Periodicals; Environmental toxicology -- Periodicals; Environmental Pollution -- Periodicals; Environmental Pollutants -- Periodicals; Environmental Monitoring -- Periodicals; Écotoxicologie -- Périodiques; Pollution -- Périodiques; 615.902
• DOI: 10.1002/tox.22561
• Languages: English
• ISSNs: 1520-4081

Abstract

Abstract: Exposure to particulate matter (PM) leads to kinds of cardiopulmonary diseases, such as asthma, COPD, arrhythmias, lung cancer, etc., which are related to PM‐induced inflammation. We have found that PM2.5 (aerodynamics diameter <2.5 µm) exposure induces inflammatory response both in vivo and in vitro. Since the toxicity of PM is tightly associated with its size and components, PM1 (aerodynamics diameter <1.0 µm) is supposed to be more toxic than PM2.5 . However, the mechanism of PM1 ‐induced inflammation is not clear. Recently, emerging evidences prove that microRNAs play a vital role in regulating inflammation. Therefore, we studied the regulation of miR‐146a in PM1 ‐induced inflammation in human lung bronchial epithelial BEAS‐2B cells. The results show that PM1 induces the increase of IL‐6 and IL‐8 in BEAS‐2B cells and up‐regulates the miR‐146a expression by activating NF‐κB signaling pathway. Overexpressed miR‐146a prevents the nuclear translocation of p65 through inhibiting the IRAK1/TRAF6 expression, and downregulates the expression of IL‐6 and IL‐8 . Taken together, these results demonstrate that miR‐146a can negatively feedback regulate PM1 ‐induced inflammation via NF‐κB signaling pathway in BEAS‐2B cells.