A guide to integrating immunohistochemistry and chemical imaging. (15th March 2018)

• Record Type: Journal Article
• Authors: Bishop, David P.; Cole, Nerida; Zhang, Tracy; Doble, Philip A.; Hare, Dominic J.
• Is Part Of: Chemical Society reviews. Volume 47:Number 11(2018)
• Page Start: 3770
• Page End: 3787
• Publication Date: 2018-03-15
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c7cs00610a
• Languages: English
• ISSNs: 0306-0012

Abstract

Abstract : A 'how-to' guide for designing chemical imaging experiments using antibodies and immunohistochemistry. Abstract : Chemical imaging provides new insight into the fundamental atomic, molecular, and biochemical composition of tissue and how they are interrelated in normal physiology. Visualising and quantifying products of pathogenic reactions long before structural changes become apparent also adds a new dimension to understanding disease pathogenesis. While chemical imaging in isolation is somewhat limited by the nature of information it can provide ( e.g. peptides, metals, lipids, or functional groups), integrating immunohistochemistry allows simultaneous, targeted imaging of biomolecules while also mapping tissue composition. Together, this approach can provide invaluable information on the inner workings of the cell and the molecular basis of diseases.