A bilayered nanoshell for durable protection of single yeast cells against multiple, simultaneous hostile stimuli. Issue 21 (18th May 2018)

• Record Type: Journal Article
• Authors: Jiang, Nan; Ying, Guo-Liang; Yetisen, Ali K.; Montelongo, Yunuen; Shen, Ling; Xiao, Yu-Xuan; Busscher, Henk J.; Yang, Xiao-Yu; Su, Bao-Lian
• Is Part Of: Chemical science. Volume 9:Issue 21(2018)
• Page Start: 4730
• Page End: 4735
• Publication Date: 2018-05-18
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c8sc01130c
• Languages: English
• ISSNs: 2041-6520

Abstract

Abstract : A bilayered nanoshell is used to encapsulate a single cell for high stability after hostile stimulation. Abstract : Single cell surface engineering provides the most efficient, non-genetic strategy to enhance cell stability. However, it remains a huge challenge to improve cell stability in complex artificial environments. Here, a soft biohybrid interfacial layer is fabricated on individual living-cell surfaces by their exposure to a suspension of gold nanoparticles andl -cysteine to form a protecting functional layer to which porous silica layers were bound yielding pores with a diameter of 3.9 nm. The living cells within the bilayered nanoshells maintained high viability (96 ± 2%) as demonstrated by agar plating, even after five cycles of simultaneous exposure to high temperature (40 °C), lyticase and UV light. Moreover, yeast cells encapsulated in bilayered nanoshells were more recyclable than native cells due to nutrient storage in the shell.