Concentration‐dependent effect of bleaching agents on the immunolabelling of interleukin‐6, interleukin‐17 and CD5‐positive cells in the dental pulp. (17th February 2018)

• Record Type: Journal Article
• Authors: Benetti, F.; Gomes‐Filho, J. E.; Ferreira, L. L.; Sivieri‐Araújo, G.; Ervolino, E.; Briso, A. L. F.; Cintra, L. T. A.
• Is Part Of: International endontic journal. Volume 51:Number 7(2018)
• Page Start: 789
• Page End: 799
• Publication Date: 2018-02-17
• Subjects: CD5 -- dental pulp -- hydrogen peroxide -- interleukin‐17 -- interleukin‐6 -- tooth bleaching; Endodontics -- Periodicals; 617.6342
• DOI: 10.1111/iej.12891
• Languages: English
• ISSNs: 0143-2885

Abstract

Abstract: Aim: To evaluate lymphocyte‐like cell activation (CD5‐positive cells) and the expression of interleukin (IL)‐6 and IL‐17 in the pulp after tooth bleaching with two concentrations of hydrogen peroxide (H2 O2 ). Methodology: The right and left maxillary molars from 40 rats were treated randomly with bleaching gel with 20% H2 O2 (BLUE group, 1 application of 50 min), 35% H2 O2 (MAXX group, three applications of 15 min), or placebo gel (control). After 2 and 30 days, the rats were killed ( n = 10), and the jaws were processed for histological and immunohistochemistry analysis of the pulp tissue. The scores of inflammation and immunolabelling (IL‐6/IL‐17) were submitted to Mann–Whitney and Kruskal–Wallis followed Dunn tests, respectively;anova tests were used for comparisons of number of CD5‐positive cells and pulp chamber area values ( P < 0.05). Results: At 2 days, 60% of specimens of the BLUE group were associated with moderate inflammation in pulp horns, and in the MAXX group with necrosis ( P < 0.05). At 30 days, the pulp was organized, and tertiary dentine was formed. The MAXX group had superior immunolabelling of IL‐17 at 2 days differing significantly from other groups ( P < 0.05). At 2 days, 90% of the specimens of the BLUE group had moderate immunolabelling of IL‐6, and 50% of the MAXX group had severe immunolabelling, both significantly different from the control ( P < 0.05). There was no significant difference between the groups at 30 days ( P > 0.05). CD5‐positive cells were present at 2 and 30 days, particularly in the bleached groups ( P < 0.05), without significant difference between time periods ( P > 0.05). Conclusions: IL‐6 and IL‐17 participated in inflammation in the pulp tissue of rats after tooth bleaching, particularly at 2 days. The immunolabelling was greater with increasing H2 O2 concentration. This process was accompanied by the prolonged activation of CD5‐positive cells.