Effects of oxidative stress induced by high dosage of dietary iron ingested on intestinal damage and caecal microbiota in Chinese Yellow broilers. Issue 4 (23rd March 2018)

• Record Type: Journal Article
• Authors: Gou, Z. Y.; Li, L.; Fan, Q. L.; Lin, X. J.; Jiang, Z. Y.; Zheng, C. T.; Ding, F. Y.; Jiang, S. Q.
• Is Part Of: Journal of animal physiology and animal nutrition. Volume 102:Issue 4(2018)
• Page Start: 924
• Page End: 932
• Publication Date: 2018-03-23
• Subjects: caecal microbiota -- Chinese Yellow broiler -- intestinal lesion -- iron -- oxidative stress; Animal nutrition -- Periodicals; Feeds -- Periodicals; 636.085
• DOI: 10.1111/jpn.12885
• Languages: English
• ISSNs: 0931-2439

Abstract

Summary: The objective of this trial was to test the effects of oxidative stress induced by a high dosage of dietary iron on intestinal lesion and the microbiological compositions in caecum in Chinese Yellow broilers. A total of 450 1‐day‐old male chicks were randomly allotted into three groups. Supplemental iron (0, 700 and 1, 400 mg/kg) was added to the basal diet resulting in three treatments containing 245, 908 and 1, 651 mg/kg Fe (measured value) in diet respectively. Each treatment consisted of six replicate pens with 25 birds per pen. Jejunal enterocyte ultrastructure was observed by transmission electron microscopy. The results showed that a high dosage of dietary iron induced oxidative stress in broilers. Dilated endoplasmic reticulum (ER), autophagosome formation of jejunal enterocytes and decreased villi were caused by this oxidative stress. Compared to the control, concentration of the malondialdehyde (MDA) in jejunal mucosa in the 908 and 1, 651 mg/kg Fe groups increased by 180% ( p < .01) and 155% respectively ( p < .01); activity of copper‐zinc superoxide dismutase (Cu/ZnSOD) increased in jejunum ( p < .01); and the concentration of plasma reduced glutathione (GSH) decreased by 34.9% ( p < .01) in birds fed 1, 651 mg/kg Fe. Gene expression of nuclear factor, erythroid‐derived 2‐like 2 ( Nrf2 ) and zonula occludens‐1 ( ZO‐1 ), in the higher dietary Fe groups was enhanced ( p < .05). Species of microbial flora in caecum increased caused by oxidative stress. The PCR‐DGGE (denaturing gradient gel electrophoresis) dendrograms revealed different microbiota (65% similarity coefficient) between the control and iron‐supplemented groups ( p < .05). These data suggest high dosage of iron supplement in feed diet can induce oxidative stress in Chinese Yellow broilers, and composition of microbiota in the caecum changed. It implied there should be no addition of excess iron when formulating diets in Chinese Yellow broilers.