Positive selection on the mitochondrial ATP synthase 6 and the NADH dehydrogenase 2 genes across 22 hare species (genus Lepus). (9th January 2018)

• Record Type: Journal Article
• Authors: Ben Slimen, Hichem; Awadi, Asma; Tolesa, Zelalem Gebremariam; Knauer, Felix; Alves, Paulo Célio; Makni, Mohamed; Suchentrunk, Franz
• Is Part Of: Journal of zoological systematics and evolutionary research. Volume 56:Number 3(2018)
• Page Start: 428
• Page End: 443
• Publication Date: 2018-01-09
• Subjects: climate -- environmental variation -- MT‐ATP6 -- MT‐ND2 -- positive selection; Animals -- Classification -- Periodicals; Zoology -- Periodicals; Evolution -- Periodicals; 578.012
• DOI: 10.1111/jzs.12204
• Languages: English
• ISSNs: 0947-5745

Abstract

Abstract: Amino acid changes in mitochondrial (mt) oxidative phosphorylation (OXPHOS) genes have been suggested as a key adaptation to environmental variation. Here, we analyzed 416 sequences of ATPase synthase 6 ( MT‐ATP6 ) and NADH dehydrogenase 2 ( MT‐ND2 ) in 22 different hare ( Lepus ) species from across a wide range of habitats and climates. We used site‐ and branch‐based methods to test for positive selection on specific codons and lineages. We found four codons in MT‐ATP6 and five in MT‐ND2 under positive selection, affecting several species lineages. We investigated the association of protein variants at each locus with climate zone, using multinomial generalized linear models (glm), including species, regions, historical introgression events, and the co‐occurring protein variant at the other locus as additional explanatory variables. A significant climate effect as based on the "Köppen climate classification" was observed for MT‐ND2 protein variants as translated from our nucleotide sequences. Moreover, MT‐ND2 protein variants were significantly affected by the co‐occurring MT‐ATP6 protein variant in the same mtDNA molecule. Contrary to the expectation for non‐recombining mitochondrial DNA molecules, the presence of an evolutionarily relatively ancestral protein variant at one locus was associated with a relatively derived protein at the other locus in the same mitochondrial molecule, respectively. The relative evolutionary status of a protein variant was evaluated according to its positions relative to the respective out‐group protein variant in a network analysis of nucleotide sequences. All our results suggest a complex effect of various climatic parameters acting on multiple mtOXPHOS genes in a co‐adaptive way, favoring combinations of ancestral and derived variants. Abstract : Site and branch‐based tests revealed positive selection on MT‐ATP6 and MT‐ND2 sequences of 22 different hare ( Lepus ) species from a wide range of habitats and climates. Moreover, a significant climate effect on the presence of MT‐ND2 protein variants was found. Our results are in line with earlier findings along a steep ecological gradient in North Africa and suggest complex effects of various climatic parameters acting on mtOXPHOS genes in a co‐adaptive way, favouring combinations of ancestral and derived protein variants.