A potential role for the silent information regulator 2 homologue 1 (SIRT1) in periapical periodontitis. (27th February 2018)

• Record Type: Journal Article
• Authors: Kudo, H.; Takeichi, O.; Hatori, K.; Makino, K.; Himi, K.; Ogiso, B.
• Is Part Of: International endontic journal. Volume 51:Number 7(2018)
• Page Start: 747
• Page End: 757
• Publication Date: 2018-02-27
• Subjects: angiogenesis -- endothelial cells -- periapical granulomas -- periapical periodontitis -- SIRT1 -- wound healing; Endodontics -- Periodicals; 617.6342
• DOI: 10.1111/iej.12894
• Languages: English
• ISSNs: 0143-2885

Abstract

Abstract: Aim: To investigate the role played by silent information regulator 2 homologue 1 (SIRT1) during angiogenesis of periapical periodontitis. Methodology: Periapical granulomas were subjected to dual‐colour immunofluorescence imaging and real‐time polymerase chain reactions assaying the expression levels of SIRT1, vascular endothelial growth factor (VEGF) and VE‐cadherin. The association between Ki‐67 and SIRT1 expression was also examined. Human umbilical vein endothelial cells (HUVECs) were treated with a combination of lipopolysaccharide and resveratrol (a SIRT1 activator) or sirtinol (a SIRT1 inhibitor); and the levels of mRNAs encoding SIRT1, VEGF and VE‐cadherin were determined. HUVEC tube formation was assayed in the presence of resveratrol or sirtinol. The Mann–Whitney U ‐test or the Tukey–Kramer test was used for statistical analysis. Results: Ki‐67‐expressing cells, including endothelial cells, lay adjacent to SIRT1‐expressing cells in periapical granulomas. In addition, SIRT1‐expressing cells were detected adjacent to VEGF‐expressing cells and VEGF‐ or VE‐cadherin‐expressing endothelial cells. SIRT1, VEGF and VE‐cadherin mRNA expression levels in periapical granulomas were significantly higher ( P = 0.0054, 0.0090 and 0.0090, respectively) than those in healthy gingival tissues. HUVECs treated with resveratrol exhibited significantly higher expression of mRNAs encoding SIRT1, VEGF and VE‐cadherin ( P = 0.0019, 0.00005 and 0.0045, respectively) compared with controls, but sirtinol inhibited such expression. Resveratrol caused HUVECs to form tube‐like structures, whilst sirtinol inhibited this process. Conclusions: These findings suggest that SIRT1 may stimulate angiogenesis in periapical granulomas by triggering the proliferation of endothelial cells and inducing VEGF and VE‐cadherin expression.