Whole‐transcriptome analysis reveals genetic factors underlying flowering time regulation in rapeseed (Brassica napus L.). (19th June 2018)

• Record Type: Journal Article
• Authors: Shah, Smit; Weinholdt, Claus; Jedrusik, Nicole; Molina, Carlos; Zou, Jun; Große, Ivo; Schiessl, Sarah; Jung, Christian; Emrani, Nazgol
• Is Part Of: Plant, cell and environment. Volume 41:Number 8(2018)
• Page Start: 1935
• Page End: 1947
• Publication Date: 2018-06-19
• Subjects: association analysis -- differentially expressed genes -- genetic mapping -- pleiotropic effects -- RNA‐seq -- vernalization -- yield; Plant physiology -- Periodicals; Plant cells and tissues -- Periodicals; Plant communities -- Periodicals; 581.105
• DOI: 10.1111/pce.13353
• Languages: English
• ISSNs: 0140-7791

Abstract

Abstract: Rapeseed ( Brassica napus L.), one of the most important sources of vegetable oil and protein‐rich meals worldwide, is adapted to different geographical regions by modification of flowering time. Rapeseed cultivars have different day length and vernalization requirements, which categorize them into winter, spring, and semiwinter ecotypes. To gain a deeper insight into genetic factors controlling floral transition in B. napus, we performed RNA sequencing (RNA‐seq) in the semiwinter doubled haploid line, Ningyou7, at different developmental stages and temperature regimes. The expression profiles of more than 54, 000 gene models were compared between different treatments and developmental stages, and the differentially expressed genes were considered as targets for association analysis and genetic mapping to confirm their role in floral transition. Consequently, 36 genes with association to flowering time, seed yield, or both were identified. We found novel indications for neofunctionalization in homologs of known flowering time regulators like VIN3 and FUL . Our study proved the potential of RNA‐seq along with association analysis and genetic mapping to identify candidate genes for floral transition in rapeseed. The candidate genes identified in this study could be subjected to genetic modification or targeted mutagenesis and genotype building to breed rapeseed adapted to certain environments. Abstract : Understanding the complex flowering time mechanism in rapeseed helps to fine‐tune the yield and to upsurge the genetic diversity. Employing transcriptome study, association analysis, and genetic mapping, we could confirm the role of 36 candidate genes in regulation of flowering time and/or yield‐related traits in rapeseed. Moreover, we found novel indications for neofunctionalization in homologs of known flowering time regulators like VIN3 and FUL .