EDTA interference in electrochemiluminescence ACTH assay. (November 2016)

• Record Type: Journal Article
• Authors: Toprak, Burak; Yalcin, Hulya; Arı, Elif; Colak, Ayfer
• Is Part Of: Annals of clinical biochemistry. Volume 53:Number 6(2016:Nov.)
• Page Start: 699
• Page End: 701
• Publication Date: 2016-11
• Subjects: ACTH -- EDTA -- plasma; Clinical chemistry -- Periodicals; Clinical biochemistry -- Periodicals; 616.075
• DOI: 10.1177/0004563216636898
• Languages: English
• ISSNs: 0004-5632

Abstract

Background: As plasma is the recommended sample type for Roche adrenocorticotropic hormone (ACTH) assay, we evaluated the effect of EDTA concentration on Cobas ACTH assay. Methods: Samples containing twofold and fourfold higher concentrations of EDTA were prepared by adding plasma to empty K2EDTA tubes and by making under-filled EDTA tubes. All measurements were performed with four replicates. Results: Increased EDTA concentration resulted in a significant decrease in ACTH concentration. Fifty-per cent-filled EDTA tube showed 19% decrease in ACTH concentration and 25% filled EDTA tube showed 50% decrease in ACTH concentration. Conclusion: We recommend that inadequately filled EDTA specimens should be rejected when using Cobas ACTH assay.