Antiproliferative and apoptosis-induction studies of 5-hydroxy 3′, 4′, 7-trimethoxyflavone in human breast cancer cells MCF-7: an in vitro and in silico approach. (4th May 2018)

• Record Type: Journal Article
• Authors: Sudha, A.; Srinivasan, P.; Kanimozhi, V.; Palanivel, K.; Kadalmani, B.
• Is Part Of: Journal of receptor and signal transduction research. Volume 38:Number 3(2018)
• Page Start: 179
• Page End: 190
• Publication Date: 2018-05-04
• Subjects: HTMF -- MCF-7 -- AO/EtBr -- Bcl-2 -- MDM2-p53 -- molecular docking; Cell receptors -- Periodicals; Cellular signal transduction -- Periodicals; 571.6
• DOI: 10.1080/10799893.2018.1468780
• Languages: English
• ISSNs: 1079-9893

Abstract

Abstract: The aim of this study was to find the efficacy of 5-hydroxy 3′, 4′, 7-trimethoxyflavone (HTMF), a flavonoid compound isolated from the medicinal plant Lippia nodiflora, in inhibiting the proliferation and inducing apoptosis in human breast cancer cell line MCF-7. The anti-proliferative effect of the compound HTMF was confirmed using MTT cytotoxicity assay. Increased apoptotic induction by HTMF was demonstrated by acridine orange/ethidium bromide (AO/EtBr) and Hoechst 33258 staining studies. The phosphatidylserine translocation, an early feature of apoptosis and DNA damage were revealed through AnnexinV-Cy3 staining and comet assay. Moreover, the significant elevation of cellular ROS was observed in the treated cells, as measured by 2, 7-diacetyl dichlorofluorescein (DCFH-DA). The mRNA expression studies also supported the effectiveness of HTMF by shifting the Bax:Bcl-2 ratio. The treatment of MCF-7 cells with HTMF encouraged apoptosis through the modulation of apoptotic markers, such as p53, Bcl-2, Bax, and cleaved PARP. In silico molecular docking and dynamics studies with MDM2-p53 protein revealed that HTMF was more potent compound that could inhibit the binding of MDM2 with p53 and, therefore, could trigger apoptosis in cancer cell. Overall, this study brings up scientific evidence for the efficacy of HTMF against MCF-7 breast cancer cells. Graphical Abstract: