Aryloxyalkanoate Dioxygenase-12 Soybean Protein Expression. Issue 1 (March 2015)

• Record Type: Journal Article
• Authors: Robinson, Andrew P.; Simpson, D. M.; Yau, Kerrm; Canada, Sarah; Johnson, William G.
• Is Part Of: Weed science. Volume 63: Issue 1(2015)
• Page Start: 229
• Page End: 234
• Publication Date: 2015-03
• Subjects: 2, 4-D, -- Glycine max (L.) Merr., soybean.; 2, 4-dichlorophenoxyacetic acid, -- 2, 4-D-tolerant soybean, -- transgenic soybean; 632.505
• DOI: 10.1614/WS-D-13-00035.1
• Languages: English
• ISSNs: 0043-1745

Abstract

Abstract : New trait technology incorporating 2, 4-D resistance in soybean is dependent upon the ability of the plant to metabolize 2, 4-D by the aryloxyalkanoate dioxygenase-12 protein (AAD-12). Our objectives were to determine AAD-12 expression during the daytime, throughout the leaf canopy, and before and after 2, 4-D treatment for the events DAS-68416-4 and DAS-21606-3. Field experiments were conducted near Wanatah, IN in 2009 and Fowler, IN in 2009, 2010, and 2011. During the daytime, total AAD-12 expression was lowest between 12:30 and 15:30, averaging 161 ng cm −2, as compared to an average of 245 ng cm −2 in the morning and 243 ng cm −2 in the evening. The youngest fully emerged trifoliate in the DAS-68416-4 event had the highest AAD-12 expression, with means ranging from 369 to 390 ng cm −2, while the older leaves maintained a lower level of expression, 171 to 211 ng cm −2 . The youngest leaves of event DAS-21606-3 had the highest level of AAD-12 expression (205 to 225 ng cm −2 ), while the level of AAD-12 was lower in older leaves (71 to 149 ng cm −2 ). In general, 2, 4-D treatments did not reduce AAD-12 expression at 3, 7, 14, and 21 days after treatment; however, in a few instances AAD-12 expression was increased or decreased by 8 to 11% after 2, 4-D treatment. Expression of AAD-12 was between 152 to 390 ng cm −2 for DAS-68416-4 and from 71 to 244 ng cm −2 for DAS-21606-3.