Novel dual-site fluorescent probe for monitoring cysteine and sulfite in living cells. Issue 38 (8th June 2018)

• Record Type: Journal Article
• Authors: Guo, Xin; Xia, Lili; Huang, Jinxin; Wang, Yiming; Gu, Yueqing; Wang, Peng
• Is Part Of: RSC advances. Volume 8:Issue 38(2018)
• Page Start: 21047
• Page End: 21053
• Publication Date: 2018-06-08
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c8ra01683f
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : A dual-site fluorescence probeLC-1 was developed for the detection of Cys and SO3 2− . Abstract : Fluorescent probes have been considered to be efficient tools for the visualization of physiological and pathological processes. Herein, a dual-site fluorescence probe denoted asLC-1 was developed for the detection of cysteine (Cys) and its metabolite SO3 2− . The probe was shown to be highly sensitive to Cys and SO3 2− with a turn-on mode fluorescence signal through two emission channels under excitations at wavelengths of 320 nm and 440 nm. Notably, theLC-1 probe was also observed to be satisfactorily sensitive to Cys and SO3 2− in the presence of other amino acids and reactive oxygen species (ROS). Meanwhile, LC-1 was shown to have low cytotoxicity and was successfully applied for imaging the metabolism of Cys in living cells.