An artificial metalloenzyme for carbene transfer based on a biotinylated dirhodium anchored within streptavidin. Issue 9 (20th April 2018)

• Record Type: Journal Article
• Authors: Zhao, Jingming; Bachmann, Daniel G.; Lenz, Markus; Gillingham, Dennis G.; Ward, Thomas R.
• Is Part Of: Catalysis science & technology. Volume 8:Issue 9(2018)
• Page Start: 2294
• Page End: 2298
• Publication Date: 2018-04-20
• Subjects: Catalysis -- Periodicals; 541.395
• DOI: 10.1039/c8cy00646f
• Languages: English
• ISSNs: 2044-4753

Abstract

Abstract : We report an artificial carbenoid transferase which combines a biotinylated dirhodium moiety within streptavidin scaffold. Abstract : We report on artificial metalloenzymes that incorporate a biotinylated dirhodium core embedded within engineered streptavidin (Sav hereafter) variants. The resulting biohybrid catalyzes the carbene insertion in C–H bonds and olefins. Chemical- and genetic optimization allows to modulate the catalytic activity of the artificial metalloenzymes that are shown to be active in the periplasm of E. coli (up to 20 turnovers).