In situ polymerization of poly(γ-benzyl-l-glutamate) on mesoporous hydroxyapatite with high graft amounts for the direct fabrication of biodegradable cell microcarriers and their osteogenic induction. Issue 20 (9th May 2018)

• Record Type: Journal Article
• Authors: Li, Linlong; Shi, Xincui; Wang, Zongliang; Wang, Yu; Jiao, Zixue; Zhang, Peibiao
• Is Part Of: Journal of materials chemistry. Volume 6:Issue 20(2018)
• Page Start: 3315
• Page End: 3330
• Publication Date: 2018-05-09
• Subjects: Materials -- Periodicals; Chemistry, Analytic -- Periodicals; Biomedical materials -- Research -- Periodicals; 543.0284
• DOI: 10.1039/c8tb00232k
• Languages: English
• ISSNs: 2050-750X

Abstract

Abstract : Through this method, PBLG- g -MHA microcarriers with particular properties could be prepared without any non-essential components, such as PLGA. Abstract : Large-scale cell culture for cell expansion in tissue engineering is currently a major focus of research. One method to achieve better cell amplification is to utilize microcarriers. In this study, different amounts of poly(γ-benzyl-l -glutamate) (PBLG) (from 11 wt% to 50 wt%) were grafted on mesoporous hydroxyapatite (MHA) by the in situ ring opening polymerization of γ-benzyl-l -glutamate N -carboxyanhydride (BLG-NCA), and biodegradable and biocompatible PBLG- g -MHA microcarriers were directly fabricated using the oil-in-water (O/W) solvent-evaporation technique for bone tissue engineering. The amount of grafted PBLG could be controlled by adjusting the feed ratio of MHA and BLG-NCA. The relationships between sphere morphology and graft amount or solution concentration were explored. Furthermore, cytological assays were performed to evaluate the biological properties of the PBLG- g -MHA microcarriers. For a solution concentration of 3% (w/v) and PBLG graft amounts of 33 wt% and 50 wt%, the microspheres could be harvested with optimal spherical shapes. In vitro cell culture revealed that the PBLG- g -MHA microspheres had favorable properties for cell proliferation and significantly enhanced the osteogenic differentiation of MC3T3-E1 cells and bone matrix formation.