Synthesis of well-dispersed Fe3O4 nanoparticles loaded on montmorillonite and sensitive colorimetric detection of H2O2 based on its peroxidase-like activity. (10th May 2018)

• Record Type: Journal Article
• Authors: Wu, Kaili; Zhao, Xin; Chen, Miaomiao; Zhang, Huawei; Liu, Zhenxue; Zhang, Xiao; Zhu, Xixi; Liu, Qingyun
• Is Part Of: New journal of chemistry. Volume 42:Number 12(2018)
• Page Start: 9578
• Page End: 9587
• Publication Date: 2018-05-10
• Subjects: Chemistry -- Periodicals; Chimie -- Périodiques; 540
• DOI: 10.1039/c8nj01190g
• Languages: English
• ISSNs: 1144-0546

Abstract

Abstract : In this study, a simple strategy for the preparation of Fe3 O4 nanoparticles (NPs) loaded on montmorillonite as a support is reported. Abstract : In this study, a simple strategy for the preparation of Fe3 O4 nanoparticles (NPs) loaded on montmorillonite as a support is reported. The Fe3 O4 –MMT nanocomposites (NCs) were demonstrated to possess peroxidase-like activity and could catalyze the oxidation of 3, 3′, 5, 5′-tetramethylbenzidine (TMB) to a blue oxidation product (oxTMB) in the presence of hydrogen peroxide. The obvious color change can be used to quantify the H2 O2 concentration using UV-vis spectroscopy. In addition, the Fe3 O4 –MMT NCs exhibit enhanced peroxidase-like activity as compared to MMT or pure Fe3 O4 NPs. Steady-state kinetics studies and a hydroxyl radical detecting assay were carried out to investigate the peroxidase-like activity and catalytic mechanism of these NCs. Under the optimized conditions, Fe3 O4 –MMT NCs are expected to be used as a good colorimetric biosensor for the detection of H2 O2, which have a linear range from 5 × 10 −5 M to 2 × 10 −4 M with a detectable H2 O2 concentration as low as 5.1 × 10 −6 M. This sensor was also successfully applied to detect H2 O2 in contact lens solution samples.