Development of hierarchical Fe3O4 magnetic microspheres as solid substrates for high sensitive immunoassays. Issue 22 (25th May 2018)

• Record Type: Journal Article
• Authors: Ma, Jiao; Song, Lingjie; Shi, Hengchong; Yang, Huawei; Ye, Wei; Guo, Xin; Luan, Shifang; Yin, Jinghua
• Is Part Of: Journal of materials chemistry. Volume 6:Issue 22(2018)
• Page Start: 3762
• Page End: 3769
• Publication Date: 2018-05-25
• Subjects: Materials -- Periodicals; Chemistry, Analytic -- Periodicals; Biomedical materials -- Research -- Periodicals; 543.0284
• DOI: 10.1039/c8tb00846a
• Languages: English
• ISSNs: 2050-750X

Abstract

Abstract : Hierarchical Fe3 O4 magnetic microspheres for sensitive immunoassays. Abstract : Improving the detection sensitivity of enzyme linked immunosorbent assay (ELISA) is of the utmost importance for meeting the demand of early disease diagnosis. In this work, a sensitive solid substrate for ELISA, i.e., hierarchical iron oxide magnetic microspheres, Fe3 O4 @mSiO2 @poly[poly(ethylene glycol) methacrylate- co -glycidyl methacrylate], was developed via a novel surface-initiated photoiniferter-mediated polymerization (SI-PIMP) strategy. The magnetic microspheres consist of a magnetic Fe3 O4 core that gives a high magnetic response, a 3D backbone, a mesoporous SiO2 middle layer, that facilitates microsphere stability and provides anchoring sites, and polymer brushes, that serve as an antifouling and oriented antibody immobilization layer. As a result, the as-prepared microspheres possess a high antibody loading capacity, an enhanced detection signal and a dramatically improved sensitivity, resulting in a 25-fold improvement over conventional ELISA solid substrates.