Ultra-sensitive fluorescent probes for hypochlorite acid detection and exogenous/endogenous imaging of living cells. Issue 57 (2nd July 2018)

• Record Type: Journal Article
• Authors: Jiang, Yin; Zheng, Guansheng; Duan, Qinya; Yang, Liu; Zhang, Jie; Zhang, Huatang; He, Jun; Sun, Hongyan; Ho, Derek
• Is Part Of: Chemical communications. Volume 54:Issue 57(2018)
• Page Start: 7967
• Page End: 7970
• Publication Date: 2018-07-02
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c8cc03963a
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : Two fluorescent probes have been developed to detect HOCl with ultra-high sensitivity and employed to image exogenous/endogenous HOCl in living cells. Abstract : Two fluorescent probes (Naph-1 andNaph-2 ), which can be prepared via a facile process, have been developed to detect hypochlorite acid (HOCl). The N, N -dimethyl thiocarbamate group quenches the fluorescence of the probes through intramolecular charge transfer (ICT). Upon reaction with HOCl in aqueous buffer, Naph-1 shows ultra-high sensitivity towards HOCl through a 4600-fold increase in fluorescence intensity, as well as a detection limit of 2.37 nM. The probes have been applied to confocal fluorescence imaging of exogenous and endogenous HOCl in living cells.