Long-wavelength TCF-based fluorescence probes for the detection and intracellular imaging of biological thiols. Issue 38 (23rd April 2018)

• Record Type: Journal Article
• Authors: Sedgwick, Adam C.; Gardiner, Jordan E.; Kim, Gyoungmi; Yevglevskis, Maksims; Lloyd, Matthew D.; Jenkins, A. Toby A.; Bull, Steven D.; Yoon, Juyoung; James, Tony D.
• Is Part Of: Chemical communications. Volume 54:Issue 38(2018)
• Page Start: 4786
• Page End: 4789
• Publication Date: 2018-04-23
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c8cc01661e
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : Two 'turn on' TCF-based fluorescence probes were developed for the detection of biological thiols (TCF-GSH andTCFCl-GSH ). Abstract : Two 'turn on' TCF-based fluorescence probes were developed for the detection of biological thiols (TCF-GSH andTCFCl-GSH ).TCF-GSH was shown to have a high sensitivity towards glutathione (GSH) with a 0.28 μM limit of detection. Unfortunately, at higher GSH concentrations the fluorescence intensity ofTCF-GSH decreased and toxicity was observed forTCF-GSH in live cells. However, TCFCl-GSH was shown to be able to detect GSH at biologically relevant concentrations with a 0.45 μM limit of detection. No toxicity was found forTCFCl-GSH and a clear 'turn on' with good photostability was observed for the exogenous addition of GSH, Cys and HCys. Furthermore, TCFCl-GSH was used to evaluate the effects of drug treatment on the levels of GSH in live cells.