A nitrocellulose membrane-based integrated microfluidic system for bacterial detection utilizing magnetic-composite membrane microdevices and bacteria-specific aptamers12. Issue 11 (16th May 2018)

• Record Type: Journal Article
• Authors: Wu, Jia-Han; Wang, Chih-Hung; Ma, Yu-Dong; Lee, Gwo-Bin
• Is Part Of: Lab on a chip. Volume 18:Issue 11(2018)
• Page Start: 1633
• Page End: 1640
• Publication Date: 2018-05-16
• Subjects: Miniature electronic equipment -- Periodicals; Combinatorial chemistry -- Periodicals; Biotechnology -- Periodicals; 543.0813
• DOI: 10.1039/c8lc00251g
• Languages: English
• ISSNs: 1473-0197

Abstract

Abstract : A new, nitrocellulose-based microfluidic system featuring Acinetobacter baumannii (AB)-specific aptamers capable of automating the bacterial detection process was reported. Abstract : Bacteria such as Acinetobacter baumannii (AB) can cause serious infections, resulting in high mortality if not diagnosed early and treated properly; there is consequently a need for rapid and accurate detection of this bacterial species. Therefore, we developed a new, nitrocellulose-based microfluidic system featuring AB-specific aptamers capable of automating the bacterial detection process via the activity of microfluidic devices composed of magnetic-composite membranes. Electromagnets were used to actuate these microfluidic devices such that the entire diagnostic process could be conducted in the integrated microfluidic system within 40 minutes with a limit of detection as low as 450 CFU per reaction for AB . Aptamers were used to capture AB in complex samples on nitrocellulose membranes, and a simple colorimetric assay was used to estimate bacterial loads. Given the ease of use, portability, and sensitivity of this aptamer-based microfluidic system, it may hold great promise for point-of-care diagnostics.