Controlled grafted poly(quaternized-4-vinylpyridine-co-acrylic acid) brushes attract bacteria for effective antimicrobial surfaces. Issue 22 (29th May 2018)

• Record Type: Journal Article
• Authors: Khan, Mashooq; Wu, Zengnan; Mao, Sifeng; Shah, Syed Niaz Ali; Lin, Jin-Ming
• Is Part Of: Journal of materials chemistry. Volume 6:Issue 22(2018)
• Page Start: 3782
• Page End: 3791
• Publication Date: 2018-05-29
• Subjects: Materials -- Periodicals; Chemistry, Analytic -- Periodicals; Biomedical materials -- Research -- Periodicals; 543.0284
• DOI: 10.1039/c8tb00702k
• Languages: English
• ISSNs: 2050-750X

Abstract

Abstract : The substrates were coated with P(Q4VP- co -AA) brushes and adsorbed GA to attract, kill and release microbes. Abstract : Copolymer poly(quaternized-4-vinylpyridine- co -acrylic acid) (P(Q4VP- co -AA)) brushes with an adsorbed amino acid (l -glutamine (GA)) were grafted onto surfaces as a means of attracting, contact-killing, and releasing bacteria. The substrates, silicon wafer (SW), glass, and PDMS, were coated with 7-octenyltrichlorosilane (7OTS), and this was followed by surface-initiated reversible addition–fragmentation transfer (SI-RAFT) polymerization. The 7OTS allowed easy control of the grafting density, and a maximum grafting density of 0.36 chains per nm 2 was obtained. The GA was initially adsorbed to the P(Q4VP- co -AA) brushes, and ultimately desorbed in a microbial environment-created concentration gradient, causing microbial movement toward the decorated surfaces. The P(Q4VP- co -AA) brushes at SW, glass, and PDMS substrates enabled the contact-killing of 90 ± 4, 93 ± 5, and 92 ± 3% of Escherichia coli and 92 ± 4, 93 ± 3, and 91 ± 4% of Staphylococcus aureus, respectively, from microbial concentrations of 1 × 10 5 CFU mL −1 and 30 min exposures. After contact-killing, these brushes completely released the microbes when they were dipped in NaCl (1 M) solution for 5 min. The ease of controlling the grafting density and substrate modification as well as the microbial attraction capability, high contact-killing efficiency, and self-sterilizing make P(Q4VP- co -AA)–GA brushes a useful material for the development of multi-functional antimicrobial substrates.