Binding studies between cytosinpeptidemycin and the superfamily 1 helicase protein of tobacco mosaic virus. Issue 34 (23rd May 2018)

• Record Type: Journal Article
• Authors: Li, Xiangyang; Chen, Kai; Gao, Di; Wang, Dongmei; Huang, Maoxi; Zhu, Hengmin; Kang, Jinxin
• Is Part Of: RSC advances. Volume 8:Issue 34(2018)
• Page Start: 18952
• Page End: 18958
• Publication Date: 2018-05-23
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c8ra01466c
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : Tobacco mosaic virus (TMV) helicases play important roles in viral multiplication and interactions with host organisms. Abstract : Tobacco mosaic virus (TMV) helicases play important roles in viral multiplication and interactions with host organisms. They can also be targeted by antiviral agents. Cytosinpeptidemycin has a good control effect against TMV. However, the mechanism of action is unclear. In this study, we expressed and purified TMV superfamily 1 helicase (TMV-Hel) and analyzed its three-dimensional structure. Furthermore, the binding interactions of TMV-Hel and cytosinpeptidemycin were studied. Microscale thermophoresis and isothermal titration calorimetry experiments showed that cytosinpeptidemycin bound to TMV-Hel with a dissociation constant of 0.24–0.44 μM. Docking studies provided further insights into the interaction of cytosinpeptidemycin with the His375 of TMV-Hel. Mutational and Microscale thermophoresis analyses showed that cytosinpeptidemycin bound to a TMV-Hel mutant (H375A) with a dissociation constant of 14.5 μM. Thus, His375 may be the important binding site for cytosinpeptidemycin. The data are important for designing and synthesizing new effective antiphytoviral agents.